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Lol trying to disguise meltdown
The fun I have on these boards is what we see with this Blake freak, he is the perfect example. That poster that just can't stand the fact I know all this stuff, I have fun watching them, how hard they work at...see see you were wrong....hahahaha!!!!!
I got into all this stuff long long ago, and it should be obvious unless you're re ed like this Blake dummy. Look at how hard he tries to erase my 50 some years of experience, it's comical. And for some reason he just can't understand yes some of us are long time fans, seen it all.
And, he will be right here again doing his.....DUH!...routine, it really is amazing.
Lol trying to disguise meltdown
Will this dummy ever figure out how badly I use him?
Just tell this weirdo....you will be ignored....and here he comes, you will have him hook, line and sinker, hahahaha!!!!!!!
Bold predictions
Alabama beats Clemson
Cam Newton wins the MVP
Steelers beat Bengals
Chiefs beat Texans
Seahawks beat Vikes
Packers beat Skins
More people will die because of that stupid Islam.
Blake will once again hump my leg.
Anyone disagree with any of that?
Lol bold.
I predict Avante will talk about me some more. Old man can't get me out of his head or off his tongue
You just force yourself on his tongue, huh? That's weird because you are probably triple the age of his usual.
The guy follows me everywhere I go and I never even talk to the sick . Just how messed up is this freak? BUT...I do have my fun with the and he's too damn stupid to see it.
For some reason the boy can't handle the fact I know all this stuff, he MUST MUST MUST do all he can to fight it, hahahahaha!!!!! Why he even cares is what gets me. Yep, the guy is ed up.
Last edited by Avante; 01-07-2016 at 11:31 AM.
Easiest money ever
Why would anyone spend a huge % of their time here following Avante around, hahaha!!!!!!!!!!!!!! Do I own this boy or what?
Check this dumb out, this is how bad he has it.
I once said...Nick Chubb will do great (RB replacing a great RB) the guy has a so so first game. Here comes this idiot.."oh yeah, well he only averages.....".....hahahaha!!!!! Nick Chubb went on to set Georgia records, he was considered to be one of the best backs in the NCAA.......crickets. That's how ed up this freak is.
10 things that amaze me.
Arizona didn't become a state until 1916.
The gorilla wasn't known about by the white world until 1847.
New Orleans and Atlanta were once in the western division of the NFL.
The Big10 has more than 10 schools.
Anyone would waste a first round pick on Johnny Manziel.
Pot was ever illegal.
Borders went out of business, I'd spend hours there.
Anyone dumb enought to think I was once a fish.
We allow Muslims in the USA.
Nobody believes in big foot, despite the fact so many have seen them.
I lied.
11. I've seen a UFO, there is something out there.
http://www.eurekalert.org/pub_releas...-tia010716.php
A single chance mutation about a billion years ago caused an ancient protein to evolve a new function essential for multicellularity in animals, according to new research co-led by a University of Chicago scientist. By conducting experiments on 'resurrected' ancestral proteins, the researchers shed light on the origin of a molecular process that allows animals to form and maintain organized tissues.
The study, published in the journal eLife on Jan. 7, 2016, is the first to experimentally describe a molecular mechanism involved in the evolution of multicellularity, and it establishes a paradigm for research in evolutionary cell biology and the origins of complex life.
"Our experiments show how biological complexity can evolve though simple, high-probability genetic paths," said the study's co-senior author Joe Thornton, PhD, professor of human genetics and ecology and evolution at the University of Chicago. "Before the last common ancestor of all animals, when only single-celled organisms existed on Earth, just one tiny change in DNA sequence caused a protein to switch from its primordial role as an enzyme to a new function that became essential to organize multicellular structures."
Few events in the history of life on Earth are as significant as the evolution of multicellular animals from single-celled ancestors. Multicellularity depends on a suite of cellular interactions and molecular functions, but almost nothing is known about how those functions evolved.
Thornton and his colleagues focused on a process called mitotic spindle orientation. To form and maintain organized tissues, cells must orient the direction in which they divide relative to their neighbors. In the flat tissues that line organs, for example, cells divide within the plane of the tissue; otherwise, malformations and cancer can result. Cells accomplish this through a structure called the mitotic spindle -- a network of protein filaments that pulls freshly duplicated chromosomes to opposite ends of the cell before it splits into two.
In cells from a broad range of animal species, the spindle is rotated relative to surrounding cells by a protein scaffold known as the guanylate kinase protein interaction domain (GK-PID). It acts as a kind of molecular carabiner by binding to two different partner molecules: an 'anchor' protein on the inside of the cell membrane that indicates the position of adjacent cells and a motor protein that pulls on mitotic spindle filaments. Once hooked together by GK-PID, the motors pull the chromosomes toward the anchors, orienting new daughter cells in line with neighboring cells.
Molecular time travel
To study how GK-PID evolved its function as a spindle-orienting carabiner, Thornton and his colleagues used ancestral protein reconstruction -- a technique Thornton's group pioneered -- to experimentally retrace the evolution of genes and proteins by working backwards through the tree of life.
Graduate students Doug Anderson and Victor Hanson-Smith first used computational methods to reconstruct the evolutionary histories of GK-PID and a related enzyme known as guanylate kinase (gk). The two proteins share similarities in sequence and structure but have different functions and histories -- GK-PID is found in only animals and their closest unicellular relatives, while gk plays a fundamental role in making the components of DNA and is universal to life.
Their analysis revealed that GK-PID evolved when the gene for the gk enzyme duplicated and then began to diverge. This event occurred before animals and their closest unicellular relatives split from other single-celled organisms, roughly a billion years ago. One copy retained its original function, but the other evolved the capacity to serve as a molecular carabiner in spindle orientation.
The team reconstructed the ancient forms of gk and GK-PID to study how this transition occurred. Working backwards from hundreds of present-day species, they used sophisticated computational methods to infer ancestral genetic sequences from the time that the GK-PID first appeared. With study co-leader Ken Prehoda at the University of Oregon, the researchers chemically synthesized the ancestral genes and inserted them into bacterial and insect cells, which produced the proteins as they existed in the distant past.
The most ancient progenitor protein, which existed just before the duplication that produced GK-PID, functioned as an enzyme. But the researchers found that they could recapitulate evolution by introducing a single mutation, which switched the protein's function, abolishing its enzyme activity and conferring the ability to act as a carabiner that could bind the anchor protein.
Remarkably, when this slightly altered version of a one billion-year-old protein was inserted into cultured insect cells -- which had their present-day GK-PID proteins disabled and therefore could not carry out spindle orientation -- the cells became able to properly rotate their spindles relative to their neighbors.
"Our experiments show that the GK-PID evolved its carabiner function early, before multicellularity itself appeared," Thornton said. "That one ancient mutation yielded a wholly new molecular function, which helped set the stage for multicellular animals to eventually evolve."
What's old is new again
The team also investigated the evolution of the anchor proteins to which GK-PID attaches. Surprisingly, these proteins first appeared in the lineage leading to animals, suggesting that GK-PID gained its ability to bind to the anchor long before the anchor itself evolved.
Why would a protein evolve the ability to bind to something that wouldn't appear for millions of years? A deeper analysis of the proteins' structural biology suggested that the answer lies in a process that Thornton calls molecular exploitation, in which a new molecule (in this case the anchor) fortuitously binds an old protein (GK-PID) because it just happens to be structurally similar to the protein's original molecular partner.
The researchers found that the ancestral GK-PID bound the anchor protein in a very similar way to how the ancestral gk enzyme bound its substrate. This region could bind both partners because the key portion of the anchor protein happened to have a similar shape and pattern of electrical charges as the ancient enzyme substrate did. The crucial mutation in the ancestral gk protein exposed the binding surface without changing it, giving the anchor protein easier access.
"It's just coincidence that the two molecules look so similar," Thornton said. "But that lucky resemblance is why a simple genetic event could cause the evolution of a molecular partnership that is now essential to the biology of complex animals."
The group's findings provide the first detailed molecular explanation for the evolution of functions involved in multicellularity and complex life. Thornton points out that many key steps in the evolution of spindle orientation remain to be reconstructed, and still more questions are unanswered concerning the evolution of other functions that made multicellularity possible. The study of GK-PID now shows one way that the emerging scientific field of evolutionary cell biology might answer those questions.
"We hope that the approach we used -- reconstructing in detail the ancient history of protein functions -- can be applied to the evolution of other key cellular processes, revealing the whole picture of multicellular life evolving from single-celled ancestors," Thornton said.
###
The study, "Evolution of an ancient protein function involved in organized multicellularity in animals," was supported by the National Ins utes of Health (R01GM104397, R01GM087457, R01GM089977) and a Howard Hughes Medical Ins ute Early Career Scientist Award to Thornton. Additional authors include Arielle Woznicka and Nicole King from the University of California, Berkeley, William Campodonico-Burnett from the University of Oregon, and Dustin Whitney and Brian Volkman from the Medical College of Wisconsin.
http://www.eurekalert.org/pub_releas...-tia010716.php
Religious freedom amazes Avante.
smh fat ol re
Dumb as a rock ain't ya slick?
The stupidest thing going on today.
The NFL Pro Bowl.
NOBODY...wants to play in this game..NOBODY~~~~~
If I've been voted in I' m faking an injury my last time on the field of the season. Getting me out of that stupidity.
Watch how many do all they can to get out of it.
I love football, love it but I haven't watched a pro bowl in ages. A game with..0...intensity, nay. I keep waiting for somebody to get seriously hurt costing them the next season, that's what it will take to dump that silliness. Sure ya vote on the all conference teams the All Pros, ya just don't play another game. Some of these guys not playing football for weeks.
Tweak in gene expression may have helped humans walk upright
http://www.sciencedaily.com/releases...0107140413.htmResearchers have identified a change in gene expression between humans and primates that may have helped give us this edge when it comes to walking upright. And they did it by studying a tiny fish called the threespine stickleback that has evolved radically different skeletal structures to match environments around the world.
Consider the engineering marvel that is your foot. Be it hairy or homely, without its solid support you'd be hard-pressed to walk or jump normally.
Now, researchers at the Stanford University School of Medicine and the HudsonAlpha Ins ute for Biotechnology in Huntsville, Alabama, have identified a change in gene expression between humans and primates that may have helped give us this edge when it comes to walking upright. And they did it by studying a tiny fish called the threespine stickleback that has evolved radically different skeletal structures to match environments around the world.
"It's somewhat unusual to have a research project that spans from fish all the way to humans, but it's clear that tweaking the expression levels of molecules called bone morphogenetic proteins can result in significant changes not just in the skeletal armor of the stickleback, but also in the hind-limb development of humans and primates," said David Kingsley, PhD, professor of developmental biology at Stanford. "This change is likely part of the reason why we've evolved from having a grasping hind foot like a chimp to a weight-bearing structure that allows us to walk on two legs."
Kingsley, who is also a Howard Hughes Medical Ins ute investigator, is the senior author of a paper describing the work that will be published online Jan. 7 in Cell. The lead author is former Stanford postdoctoral scholar Vahan Indjeian, PhD, now head of a research group at Imperial College London.
...
How about this so you'll shut the up?
Yep, we all evolved from a lesser whatever, there is no God.
Ok?
If ya saw the Alabama vs Clemson game last night ya saw the huge difference between black and white. There's that black QB running all over the place extending plays, while that white QB is getting sacked left and right.
There it is in....black and white..ha~~~
Country music is 99.99% white, then there's....
Last edited by Avante; 01-12-2016 at 02:42 PM.
I met Carlos Santana in a little bar in Hunters Point, met Steve Perry at the El Ranchero (bar) in Hanford. Met Jan Michael Vincent at a party in Lemoore, a little west of Hanford.
So there I am down at Judy's church helping them move some stuff, she was there with her mom. When in walked this little guy, his mom went to the church and was a friend of Judy's mom. So we get introduced.
When I get a chance.
Me...who is that guy (since he hugged Judy)
Judy...Barbara's son, (as if I know who Barbara is) He plays in a band.
Me...ya mind if I talk some music with him?
Judy...go ahead.
When I get a chance.
Me...so ya play in a band, what....rock?
Marty....we play the blues.
(Judy and her mom just roll their eye, it was...."oh great!"
Me...well I'll be damn, I'm kind of a blues historian.
Marty....me too, love it.
Me...cool~~~ So who is your favorite?
Marty....Blind Willie Johnson.
Me...(yep, he's the real deal)....John the Revelator.
Marty....Soul of a Man.
So we talked them old blues for about a half hour (Judy and her mom....."oh great") The guy really knew the music. Then he starts taking about playing with Kim Wilson, Mark Hummell, James Harman, Nathan James......wow!!!! I'd forgot his name, and I couldn't ask (ya know how that is). So once he left....
Me...what was his name?
Judy...Marty Dodson.
I just....well I'll be damn, ya see Marty Dodson is consdered to be one of the great blues drummers playing today.
Last edited by Avante; 01-21-2016 at 03:45 PM.
Amazing sound.
Muslims Threaten Peace
If one were to study the terrorist reports in any newspaper ever, they would see that almost every case of terrorism committed are by Muslims. If they were to be stuck in the Middle East, they would be unable to harm the rest of the population of the world. Jihads are the number one threat world peace. Anyway, the Muslims want to be near Mecca. Why not let them all go and stay?
pedo is a form of terror.
are you a muslim?
Researchers have identified a change in gene expression between humans and primates that may have helped give us this edge when it comes to walking upright. And they did it by studying a tiny fish called the threespine stickleback that has evolved radically different skeletal structures to match environments around the world.
Consider the engineering marvel that is your foot. Be it hairy or homely, without its solid support you'd be hard-pressed to walk or jump normally.
Now, researchers at the Stanford University School of Medicine and the HudsonAlpha Ins ute for Biotechnology in Huntsville, Alabama, have identified a change in gene expression between humans and primates that may have helped give us this edge when it comes to walking upright. And they did it by studying a tiny fish called the threespine stickleback that has evolved radically different skeletal structures to match environments around the world.
"It's somewhat unusual to have a research project that spans from fish all the way to humans, but it's clear that tweaking the expression levels of molecules called bone morphogenetic proteins can result in significant changes not just in the skeletal armor of the stickleback, but also in the hind-limb development of humans and primates," said David Kingsley, PhD, professor of developmental biology at Stanford. "This change is likely part of the reason why we've evolved from having a grasping hind foot like a chimp to a weight-bearing structure that allows us to walk on two legs."
Kingsley, who is also a Howard Hughes Medical Ins ute investigator, is the senior author of a paper describing the work that will be published online Jan. 7 in Cell. The lead author is former Stanford postdoctoral scholar Vahan Indjeian, PhD, now head of a research group at Imperial College London.
...
Let's break that down little fella.
Ya walk in a bar and here they come begging you to pick her. So ya buy her some drinks, she begs you to take here to a hotel. And if you don't she will get totally pissed off.
Boy, talk about terror....hahaha!!!!!!!!!!
How many dumb s do we have here. wow! No clue of any kind, hahahaha!!!!!!!!!!!!!!!
I post on a few of these boards, I gotta tell ya, there are more idiots in Texas than the rest of the world combined, dead serious.
World...of course sailors pick up bar girls....ya think?
Texas...all those sailors were pedos.
Hahahahaha!!!!!!!!!!!!!!!!!!
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