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  1. #17301
    my unders, my frgn whites pgardn's Avatar
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    Maybe graduate from micro er plates and readers to something more interesting and more complex, like MS/MS.
    you’re the only one talking about plates you fool.
    i’ve said nothing about them it’s basically minor league except it’s important what they’re made out of so you can coat them properly.

    but now we’re talking about the basics here how to make anybody show up
    you have no clue as to what is even difficult and easy you just think you can throw out terms and intimidate people.

    got the wrong guy
    sorry bud

  2. #17302
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    you’re the only one talking about plates you fool.
    i’ve said nothing about them it’s basically minor league except it’s important what they’re made out of so you can coat them properly.

    but now we’re talking about the basics here how to make anybody show up
    you have no clue as to what is even difficult and easy you just think you can throw out terms and intimidate people.

    got the wrong guy
    sorry bud
    Make anybody show up? Who did you invite?

    Did the mass spec suggestion scare you?

  3. #17303
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    I'd be happy to teach you about it if you don't know enough to discuss it.

  4. #17304
    my unders, my frgn whites pgardn's Avatar
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    You keep grading your own paper here

    I'm a professional. Do you think I am going to go into detail about that's 2nd nature to me? I gave you enough to know this isn't a novel concept. So now explain what you developed.
    You and I both know you’re a hack
    I am by no means a master scientist but I know a of a lot more than you do especially about this stuff

    No you’re not gonna go into any detail because you can’t and it doesn’t require detail if you know the stuff
    you could simply just state answers.

    and I never really want to talk about any of this on this board
    but you just so agreeably asked to be punched in the face
    all in search of your W it’s a beautiful thing

  5. #17305
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    Make anybody show up? Who did you invite?

    Did the mass spec suggestion scare you?
    yeah I try to get out of that one if I was you as well

    mr. protien

  6. #17306
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    You and I both know you’re a hack
    I am by no means a master scientist but I know a of a lot more than you do especially about this stuff

    No you’re not gonna go into any detail because you can’t and it doesn’t require detail if you know the stuff
    you could simply just state answers.

    and I never really want to talk about any of this on this board
    but you just so agreeably asked to be punched in the face
    all in search of your W it’s a beautiful thing
    I gave you the answers. If you knew anything you'd know that when I said compe ive and sandwich that binding sites are important. You only count Eu. You don't count the antibody. You never actually see the antibody. You just know the binding makes it so that every time you count Jack, you know Jill was there as well so in effect you counted Jill. If Jill is invisible, you can only see Jack, then you can only count Jill when you see Jack.

    You don't know Jack.

  7. #17307
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    yeah I try to get out of that one if I was you as well

    mr. protien
    "but now we’re talking about the basics here how to make anybody show up" -You

  8. #17308
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    "but now we’re talking about the basics here how to make anybody show up" -You
    yep explain it

    and then after that explain why it’s really difficult

    especially if you have new very sensitive techniques of detecting the complex

  9. #17309
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    Really running from the mass spec discussion, scientist guy.

  10. #17310
    my unders, my frgn whites pgardn's Avatar
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    Either read or deflect, I say you deflect, it’s your go to

  11. #17311
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    yep explain it

    and then after that explain why it’s really difficult

    especially if you have new very sensitive techniques of detecting the complex
    I haven't read any article you posted. I don't need to.

    It's not difficult. People do it all day every day. If you have techniques (new and sensitive are "ooh and ahh" words that mean nothing in science) then it can't be that difficult. Maybe you have to hand pipette, maybe you have to spin it down, maybe you have to incubate it for a long time, maybe it has a high que time, maybe maybe maybe.

    If you want specific discussions, maybe stop being so evasive and just say you were part of a study group in school.

  12. #17312
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    Really running from the mass spec discussion, scientist guy.
    mass spec?

    why the fck would you even bring that up?
    totally off subject.

    oh DMC knows a machine!

  13. #17313
    my unders, my frgn whites pgardn's Avatar
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    You said "anybody".
    no I said antibody and my voice recognition on my phone turned it to anybody

    what’s your excuse for protien Ms Crutchfield?

    damn straight you better get off topic

  14. #17314
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    mass spec?

    why the fck would you even bring that up?
    totally off subject.

    oh DMC knows a machine!
    Machine? lol

    Scientist calling an instrument a machine

  15. #17315
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    I haven't read any article you posted. I don't need to.

    It's not difficult. People do it all day every day. If you have techniques (new and sensitive are "ooh and ahh" words that mean nothing in science) then it can't be that difficult. Maybe you have to hand pipette, maybe you have to spin it down, maybe you have to incubate it for a long time, maybe it has a high que time, maybe maybe maybe.

    If you want specific discussions, maybe stop being so evasive and just say you were part of a study group in school.
    it’s a complex if the antibody binds with something fat head.

  16. #17316
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    Machine? lol

    Scientist calling an instrument a machine
    oh my God it’s just horrible!
    another deflection keep getting off subject old man

    just say a word any word uh uh .... flask! You been hitting it hard

  17. #17317
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    it’s a complex if the antibody binds with something fat head.
    "and then after that explain why it’s really difficult" -you

    You said it's really difficult. I know what a complex is. You seem to have a complex yourself.

  18. #17318
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    I gave you the Jack and Jill analogy. I know you wanted technical terms to feel special but you're not at that level yet.

  19. #17319
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    "and then after that explain why it’s really difficult" -you

    You said it's really difficult. I know what a complex is. You seem to have a complex yourself.
    it is really difficult, but it’s very easy to explain why it’s difficult dumb ass

    it takes three words
    And if you understand antibodies, those three words will be very meaningful

  20. #17320
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    it is really difficult, but it’s very easy to explain why it’s difficult dumb ass

    it takes three words
    And if you understand antibodies, those three words will be very meaningful
    So you go from pretending I was confusing "complex" with "difficult" to this ?

    Just stop. You're one of those "this is how I learned it" guys, aren't you?

    I already showed you that I know how ELISA works. I know more about it than you I'm sure. You still haven't said about what you did to develop it even after you bragged about doing so.

  21. #17321
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    I gave you the Jack and Jill analogy. I know you wanted technical terms to feel special but you're not at that level yet.
    sure you did
    you were really awful at pretending

  22. #17322
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    So you go from pretending I was confusing "complex" with "difficult" to this ?

    Just stop. You're one of those "this is how I learned it" guys, aren't you?

    I already showed you that I know how ELISA works. I know more about it than you I'm sure. You still haven't said about what you did to develop it even after you bragged about doing so.
    You read up on it and by doing so showed me you really don’t understand it
    and that’s how we detect charlatans

    to the board I must apologize for all this drivel.
    but this pompous ass we know as DMC is a fake

    Night night DMC
    you and I know what you are.
    and it’s perfect for these times.

  23. #17323
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    California’s sudden e in COVID-19 is a disturbing warning for the whole country

    there were 2,565 new cases in California on May 25 —

    breaking the previous one-day record from May 5th, when 2,546 new cases were reported across the state.

    the state as a whole is still seeing a steady increase, particularly in the Bay Area and Los Angeles County. Indeed, Los Angeles County, despite seeing its biggest e to date this week,

    is moving forward with various activities reopening.

    Because California is seen as a political and public health leader in the country,

    the sharp rise in cases is a harbinger for what is likely

    to happen in other states that follow the Golden State’s lead.

    https://www.rawstory.com/2020/05/cal...whole-country/



  24. #17324
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    Called it

    I said uk was gonna make Italy look like a weekend in the Ozarks


  25. #17325
    Take the fcking keys away baseline bum's Avatar
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    Called it

    I said uk was gonna make Italy look like a weekend in the Ozarks

    And we're following in their lead...

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