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Technique fathead.
you can’t make a standard curve against a color change, it’s a yes or no.
(qualitative)
Seriously?
"we used to just look at the micro er plate and see the color change, now we have a plate reader"
Wow the insane level of science. I just can't take no more. Next you're going to tell me there are automatic plate washers!
Technique fathead.
you can’t make a standard curve against a color change, it’s a yes or no.
(qualitative)
Oh Jesus Christ... are you really that impressed with a fluorometer, with the fact that things fluoresce will give off a particular wavelength during a dark period of a rapid flashlamp action and be picked up by a PMT??
That's all I'm giving you.
ELISA is not impressive.
Plate reader...
like this is nothing and it gets much better than that:
it’s great to pull that fish in, have him read, and then flunk because he does not know it.
isnt contagious just another word for magic?
oh my yes...
how could you possibly get an antibody to Fluoresce?
You've said basically nothing. You're talking about a plate reader, are you not? Vs eyeballing the plate (qualitative)?
only when it disappears
Keep reading.
you’re not close to done because you don’t know it.
again how do you get an antibody to Fluoresce?
keep reading
You'd have to attach a tracer! Maybe Eu? So your sandwich or compe ive assay can be counted? Just a guess.
I don't need to read anything.
yes you do. Big chap.
same question again answer it
You're a clown. I told you how it works, a flash lamp and read by a photomultiplier tube. Basic fluorometer. Add some high voltage and some lenses, a feedback loop for flash lamp control to avoid saturation, control dark counts with the proper enclosure, basic ROTM plate reader (minus the robotics).
Plate readers are kindergarten level .
you’re getting into the physics and off the biology.
wtf is a tracer?
Old man is reading right now...
I know the biology. If I know the physics then by default I'd have to know the biology. Anyone can google how tracers work. I already explained it with the compe ive vs sandwich comment.
we haven’t even got to the reading how the do you get an antibody to show itself
kindergarten level....you’re such a pompous ass. And again it’s because you don’t know what the you’re talking about
you are absolutely wrong.
do you think knowing the physics the simplest stuff of all with hardly any variables gives you knowledge of something as complex as biology?
you’ve got to be kidding!
and yeah you’re wrong, again.
but keep going on man
you can continue to show your ignorance, to me anyway
Keep reading
Show itself? That doesn't happen. You have to actually fluoresce it unless you're back to eyeballing.
Maybe graduate from micro er plates and readers to something more interesting and more complex, like MS/MS.
DMC
The stubborn old man getting ravaged by his own ignorant persistence
We will do this yet again and bore the out of the board.
how many times have you gotten baited into looking stupid because of a superiority complex? Got to have a W. The board is here to slice you up because you gotta have it.
i’ll be here for you again for about the fifth time.
you really need to stick to the guns.
Infectious diseases are hard!
You keep grading your own paper here
I'm a professional. Do you think I am going to go into detail about that's 2nd nature to me? I gave you enough to know this isn't a novel concept. So now explain what you developed.
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